Sugarcane white leaf disease caused by phytoplasma is a serious problem for sugarcane production in Thailand. The pathogen can mainly be transmitted by insect vectors such as leafhoppers, and through seed cane. Planting of disease free seed cane is an important method for reducing the disease. However, the plants may be re-infected by insect vectors. Therefore, the disease free seed cane utilization to decrease the disease was evaluated during 2006 - 2011. Sugarcane white leaf disease free seedlings were produced by apical meristem culture technique with phytoplasma detection by nested PCR method. The Seedlings were transplanted and multiplied in sugarcane planting areas mainly in Northeast of Thailand. Sugarcane white leaf phytoplasma incidence was... |